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目的:研究黄花蒿发根的生长形态和部分次生代谢物特征。方法:比较发根和未转化根的形态差异,测定生长调节剂如萘乙酸(NAA)和赤霉素(GA3)对根生长的影响,分析了发根和未转化根的部分次生代谢物的含量特征。结果:低浓度NAA(0.01～0.05 mg.L-1)能明显促进发根侧根和次侧根的形成,对未转化根则无显著作用,高浓度NAA(0.1 mg.L-1)抑制发根的生长,而对未转化根的生长起促进作用。未转化根中不含有青蒿素,而在发根中检测到较高含量的青蒿素(0.64 mg.g-1),未转化根中青蒿乙素含量较高(1.24 mg.g-1)。在未转化根和发根中,青蒿黄酮chrysoplenetin含量相当,而发根中另一种黄酮化合物artemitin含量(0.76 mg.g-1)是未转化根的3.5倍。结论:黄花蒿发根具有较独特的生长和次生代谢特征。
Objective: To study the growth morphology and some secondary metabolites of A. annua hairy root. Methods: The morphological differences of roots and untransformed roots were compared. The effects of growth regulators such as NAA and GA3 on the growth of roots were analyzed. Some secondary metabolites of root and untransformed roots were analyzed. Content characteristics. Results: Low concentration of NAA (0.01-0.05 mg.L-1) could significantly promote the formation of lateral root and minor root, but had no effect on untransformed root. NAA (0.1 mg.L-1) Of the growth, while the growth of non-transformed roots play a catalytic role. Artemisinin was not found in the untransformed roots, whereas higher levels of artemisinin (0.64 mg.g-1) were detected in the hairy roots, and the content of the artichoke B in the untransformed roots was higher (1.24 mg.g- 1). Artemisin flavone chrysoplenetin was equivalent in untransformed roots and hairy roots, while artemitin content (0.76 mg.g-1), another flavonoid in hairy roots, was 3.5 times more than that in untransformed roots. Conclusion: The root of A. annua has more unique growth and secondary metabolic characteristics.