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建立了血浆中盐酸奈福泮的HPLC测定法。血浆样品采取液 液两步提取后 ,以ODS HypersilC18为固定相 ,35 %乙腈∶6 5 % 0 .0 1mol/L庚烷磺酸钠 (pH =3 .0 )为流动相。紫外检测波长 2 15nm。最低检测浓度为 2 .5ng/ml,线性范围 2 .5～ 5 0 0 .0ng/ml(r =0 .9995 ) ,四种浓度的回收率大于 90 % ,日间日内变异系数小于15 %。
A HPLC method was established for the determination of Nefopam hydrochloride in plasma. Plasma samples were taken two steps of liquid-liquid extraction to ODS HypersilC18 as the stationary phase, 35% acetonitrile: 6.5% 0 1mol / L sodium heptane sulfonate (pH = 3.0) as the mobile phase. UV detection wavelength 2 15nm. The minimum detectable concentration was 2.5ng / ml and the linear range was 2.5-500.0ng / ml (r = 0.9995). The recoveries of the four concentrations were over 90% and the intraday variability was less than 15%.