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为探求CNTF(睫状神经营养因子)基因的A/G多态位点与我国优秀单板U型场地运动员运动能力(尤其是力量素质)的相关性。文章选择20名优秀单板U型场地运动员和115名普通无专业训练的大学生作为研究对象,采用PCR-RFLP实验法测试CNTF基因的A/G多态位点,并用基因平衡定律测试结果,用基因平衡定律(H-W)检验,组间基因型与等位基因分布采用卡方检验方法。结果显示:对照组等位基因频率为A=18.7%,G=81.3%,基因型频率为A/A=6.1%,A/G=25.2%,G/G=68.7%;实验组等位基因频率为A=17.5%,G=82.5%,基因型频为A/A=10%,A/G=15%,G/G=75%。说明CNTF三种基因型分布频率的趋势为GG型>AG型>AA型,等位基因无差异(P>0.05)。提示CNTF基因A/多态性不能作为中国技能主导类优秀滑雪运动员选材的指标之一,但GG基因型所占比例大。
In order to explore the relationship between the A / G polymorphic loci of CNTF (ciliary neurotrophic factor) gene and the athletic ability (especially strength) of excellent veneer U-shaped field athletes in China. In this study, 20 excellent U-spot athletes and 115 normal non-professional college students were selected as experimental subjects. The A / G polymorphic loci of CNTF gene were tested by PCR-RFLP. The results of gene equilibrium test Gene equilibrium (HW) test, genotype and allele distribution between groups using chi-square test. The results showed that the frequency of allele in control group was A = 18.7%, G = 81.3%, the genotype frequency was A / A = 6.1%, A / G = 25.2% and G / The frequencies were A = 17.5%, G = 82.5%, genotype frequencies were A / A = 10%, A / G = 15% and G / G = 75%. The trend of distribution frequency of three genotypes of CNTF was GG> AG> AA, with no difference in alleles (P> 0.05). It is suggested that CNTF gene A / polymorphism can not be used as one of the indexes for selecting the best skier in China, but the proportion of GG genotype is large.