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目的：通过建立白细胞介素１０（ＩＬ－１０）的重组逆转录病毒载体基因转移系统，观察ＩＬ－１０对脂多糖（ＬＰＳ）诱导的大鼠肾小球系膜细胞（ｇｌｏｍｅｒｕｌａｒｍｅｓｅｎｇｉａｌｃｅｌ，ＧＭＣ）中细胞因子的产生及其基因表达的影响。方法：通过构建的重组逆转录病毒载体ｐＬＸ（ＩＬ－１０）ＳＮ将外源基因ＩＬ－１０转移至大鼠ＧＭＣ：（１）应用聚合酶链反应（ＰＣＲ），反转录聚合酶链反应（ＲＴ－ＰＣＲ）和ＥＬＩＳＡ检测ＩＬ－１０基因的整合和表达；（２）以ＲＴ－ＰＣＲ观察ＩＬ－１０基因转移对ＬＰＳ诱导的ＧＭＣ肿瘤坏死因子－α（ＴＮＦ－α）的ｍＲＮＡ表达的影响，以ＥＬＩＳＡ测定白细胞介素－１β（ＩＬ－１β）和ＴＮＦ－α的蛋白质表达。结果：外源性ＩＬ－１０基因已整合到靶细胞染色体ＤＮＡ并有效地表达，它能抑制ＬＰＳ诱生ＧＭＣ过度产生ＩＬ－１β，ＴＮＦ－α。结论：外源性ＩＬ－１０基因可以转移到ＧＭＣ并稳定表达，它能抑制ＧＭＣ炎症效应中细胞因子的产生及其基因表达。
OBJECTIVE: To investigate the effect of IL-10 on glomerular mesangial cells (GMC) induced by lipopolysaccharide (LPS) in rats by establishing recombinant retroviral vector gene transfer system of interleukin 10 (IL-10) The production of factors and their effects on gene expression. Methods: The exogenous gene IL-10 was transferred to rat GMC via the constructed retroviral vector pLX (IL-10) SN. (1) The expression of IL-10 was detected by polymerase chain reaction (2) The effect of IL-10 gene transfer on mRNA expression of tumor necrosis factor-α (TNF-α) induced by LPS in GMCs was observed by RT-PCR, The protein expression of interleukin-1β (IL-1β) and TNF-α was measured by ELISA. Results: The exogenous IL-10 gene has been integrated into the chromosomal DNA of target cells and effectively expressed. It can inhibit LPS-induced GMC overproduction of IL-1β and TNF-α. CONCLUSION: Exogenous IL-10 gene can be transferred to GMC and stably expressed. It can inhibit the production of cytokines and its gene expression in GMC inflammatory effect.