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目的:分析银翘解毒片中绿原酸的含量,为银翘解毒片的质量标准建立提供参考。方法:采用反相高效液相色谱法,Alltech C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈(A)-0.2%醋酸水(B)系统梯度洗脱;流速为1.0 mL.min-1;检测波长280 nm;柱温为室温。结果:绿原酸进样量在0.98~12.25μg范围内线性关系良好(r=0.999 9),测得平均回收率为99.8%,RSD为0.07%。结论:方法准确,结果稳定可靠,可用于银翘解毒片的质量评价。
Objective: To analyze the content of chlorogenic acid in Yinqiaojiedu tablets and to provide reference for the establishment of Yinqiaojiedu tablets’ quality standards. Methods: A RP-HPLC method was used on Alltech C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile (A)-0.2% acetic acid water (B) gradient elution; the flow rate was 1.0 mL. Min-1; detection wavelength 280 nm; column temperature room temperature. RESULTS: The linearity of the chlorogenic acid injection ranged from 0.98 to 12.25 μg (r=0.999 9). The average recovery was 99.8% and the RSD was 0.07%. Conclusion: The method is accurate and the result is stable and reliable. It can be used to evaluate the quality of Yinqiaojiedu tablets.