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作者在本文介绍了采用特制的玻璃小针经胸接种蚊虫的技术研究四型登革病毒在白纹伊蚊中的复制情况。所用的白纹伊蚊为檀香山株;四型登革原型病毒分别为夏威夷株、新几内亚C株、H_(87)株和H_(241)株。病毒原液多由白纹伊蚊经胸接种制得。即感染蚊虫后,在32℃经7天,以冷冻杀死,并在pH7.4含有30%灭活小牛血清的磷酸缓冲盐水或不稀释的灭活小牛血清中研磨,再经1575g离心30分钟,取上清液分置于小瓶中,并浸在酒精和固体二氧化碳混合液中快速冰冻。测定病毒在蚊虫中复制曲线时,即取此病毒液定量经口或经胸感染白纹伊蚊后,在温度32℃、相对湿度65～75%下饲
In this article, we describe the technique of transinverting mosquitoes with specially prepared small glass needles to study the replication of the four dengue viruses in Aedes albopictus. The Aedes albopictus used was Honolulu strain. The four dengue virus strains were Hawaii strain, New Guinea C strain, H_ (87) strain and H_ (241) strain. Most of the virus stock solution by the Aedes albopictus inoculation obtained. That is, after being infected with mosquitoes, they were killed by freezing at 32 ° C for 7 days and ground in phosphate buffered saline, pH 7.4, containing 30% inactivated calf serum or undiluted, inactivated calf serum and centrifuged again at 1575 g After 30 minutes, the supernatant was separated into vials and quickly frozen in a mixture of alcohol and solid carbon dioxide. Determination of virus replication curve in mosquitoes, which take the virus quantitative oral or trans-thoracic infection Aedes albopictus, at a temperature of 32 ℃, relative humidity of 65 to 75% feeding