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目的:探讨去乙酰化酶SIRT1基因多态性,与长期进行不同方式有氧运动的老年人高胆固醇血症的关联性。方法:在上海市不同社区发放调查问卷,选取323名长期进行不同方式有氧运动锻炼的老年人及无长期习惯性体育锻炼的老年人60名。采集空腹静脉血,用胆固醇氧化酶法对总胆固醇含量进行测定,根据测定结果分为高胆固醇血症组(205人)和胆固醇水平正常组(178人)。应用基质辅助激光解吸附电离飞行时间质谱检测技术对SIRT1基因多态性进行检测。结果:高胆固醇血症组和胆固醇水平正常组之间SIRT1基因rs3758391位点基因型分布存在显著统计学差异(P<0.05)。携带SIRT1变异基因的个体,患高胆固醇血症的危险性较低,比值比(Odd ratio,OR)为0.559(95%CI:0.344-0.909)。多因素逻辑回归分析结果显示,SIRT1野生基因型个体,长期进行游泳锻炼的受试者表现出较低的患病风险(P<0.008)。结论:SIRT1基因rs3758391位点TT基因型可能是高胆固醇血症的易感因素。对于TT基因型个体,相比乒乓球、广场舞和太极拳,游泳锻炼可能是改善高胆固醇血症的最佳运动方式。
OBJECTIVE: To investigate the association of SIRT1 gene polymorphism with hypercholesterolemia in elderly patients with long-term aerobic exercise. Methods: Questionnaires were distributed in different communities in Shanghai. Totally, 323 elderly people who exercised in different ways for a long time and 60 elderly people who did not have long-term habitual physical exercise were selected. The fasting venous blood was collected and the total cholesterol content was determined by cholesterol oxidase method. According to the results, the patients were divided into hypercholesterolemia group (205 persons) and normal cholesterol group (178 persons). SIRT1 gene polymorphism was detected by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry. Results: The genotype distribution of rs3758391 in SIRT1 gene was significantly different between hypercholesterolemia group and normal cholesterol group (P <0.05). Individuals carrying the SIRT1 variant had a lower risk of developing hypercholesterolemia with odds ratios (ORs) of 0.559 (95% CI: 0.344-0.909). Multivariate logistic regression analysis showed that individuals with SIRT1 wild type had a lower risk of developing long-term swim training (P <0.008). Conclusion: The TT genotype of rs3758391 in SIRT1 gene may be a predisposing factor for hypercholesterolemia. For individuals with TT genotype, swimming exercise may be the best way to improve hypercholesterolemia compared to table tennis, square dance and Tai Chi.