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为明确山东地区甜菜夜蛾对高效氯氰菊酯抗性水平,采用竞争性特异等位基因PCR方法,检测甜菜夜蛾钠离子通道基因片段上L1014F的突变频率。结果表明,敏感种群个体中均未发现突变个体;田间章丘种群92%个体具有L1014F突变,其中64%为L1014F突变纯合子,28%为L1014F突变杂合子。经高效氯氰菊酯汰选5代后的抗性种群检测个体中均未发现敏感型纯合子。安丘种群的L1014F突变等位基因频率最高,为85%,泰安、滕州和滨州种群分别为50%、43.33%和30%,相关性分析表明甜菜夜蛾L1014F突变等位基因频率与对高效氯氰菊酯不敏感度呈线性正相关(Y=1.106x+0.079,R2=0.93)。
In order to clarify the resistance of beta-cypermethrin to beet armyworm in Shandong Province, the mutation frequency of L1014F in the S. japonicum sodium ion channel gene fragment was detected by competitive specific allele PCR. The results showed that no mutant individuals were found in susceptible individuals; 92% of individuals in the Zhangqiu population had L1014F mutations, of which 64% were L1014F mutant homozygotes and 28% were L1014F mutant heterozygotes. Sensitive homozygotes were not detected in all the resistant populations tested after 5 generations of beta-cypermethrin. The frequency of L1014F mutation allele in Anqiu population was the highest, 85%, while that in Taian, Tengzhou and Binzhou was 50%, 43.33% and 30%, respectively. Correlation analysis showed that the allele frequency of L1014F mutation was almost the same as that of alpha-cypermethrin Non-sensitivity was linearly positively correlated (Y = 1.106x + 0.079, R2 = 0.93).