论文部分内容阅读
为了观察卵透明带抗独特型抗体免疫抗生育中ＩＬ２、ＩＬ４两种细胞因子含量的变化，分别用猪卵透明带（ＰＺＰ）抗原、鼠抗ＰＺＰ单克隆抗体（１７Ｄ３ｍＡｂ）亲和提纯的透明带抗原和ＰＺＰ的抗独特型抗体（Ａｂ２）免疫小鼠，通过脾细胞体外诱生，测上清液中的ＩＬ２和ＩＬ４。结果发现Ａｂ２免疫组主要介导的是Ｔｈ２型免疫反应，而ＰＺＰ免疫组和１７Ｄ３ｍＡｂ靶抗原免疫组ＩＬ２水平明显升高，可能介导的是Ｔｈ１型免疫反应。结果提示Ａｂ２所模拟的抗原决定簇在ＺＰ免疫避孕的研究中是有前途的。
In order to observe the changes of the IL-2 and IL-4 cytokines in the immunobestation with anti-idiotypic antibody of zona pellucida, the zymozoon zona pellucida (PZP) antigen, mouse anti-PZP monoclonal antibody (17D3 mAb) Purified zona pellucida antigen and anti-PZP anti-idiotypic antibody (Ab2) mice were immunized spleen cells in vitro, measured in the supernatant of IL 2 and IL 4. The results showed that Ab2 immune group mainly mediated by Th2 immune response, and PZP immunization group and 17D3 mAb target antigen immunohistochemistry IL 2 levels were significantly elevated, may be mediated by the Th1-type immune response. The results suggest that the antigenic determinants of Ab2 are promising in the study of ZP immunocontraception.