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以斑点分子杂交技术检测了377份山东省麻鸭血清DHBV-DNA。结果DHBV-DNA阳性率29.97%,以RPHA检测血清中DHBsAg,阳性率32.17%,两法的一致性93.38%。对鸭肝组织病理观察,42份DHBV-DNA阳性者中,肝组织病变率为71.4%,39份阴性者中病变率为33.77%,两者差异有显著性,但未见特异性炎症改变。电镜下见有典型DHBV-DNA及DHBsAg颗粒。提示山东鸭群虽对DHBV有较高的感受性,但在肝组织内未发现病毒性肝炎特异性病理改变。
377 samples of DHBV-DNA from Shandong ducklings were detected by dot blot hybridization. Results The positive rate of DHBV-DNA was 29.97%. The positive rate of DHBsAg in serum was 32.17% by RPHA. The consistency of the two methods was 93.38%. Pathological observation of duck liver tissue showed that the rate of liver tissue lesions was 71.4% in 42 DHBV-DNA positive cases and 33.77% in 39 negative cases, the difference was significant but no specific inflammatory changes were observed. Electron microscope see a typical DHBV-DNA and DHBsAg particles. Although the duck population in Shandong Province has a higher susceptibility to DHBV, but no specific pathological changes in liver tissue found in viral hepatitis.