论文部分内容阅读
目的:研究细胞间粘附因子1(ICAM1)在着床期外周血淋巴细胞(PBLC)、子宫内膜及蜕膜细胞(EC/DC)中的不同表达特点及动态变化规律。方法:以着床期小鼠为动物模型,细胞分析采用单抗免疫荧光标记及多参数流式细胞术分析技术。结果:发现ICAM1在PBLC、EC/DC中的表达均存在明显的动态变化;其中ICAM1的表达在PBLC中于妊娠第2天(D2)达最低水平,而在EC/DC中于妊娠第4天(D4)降至最低。结论:ICAM1在PBLC及EC/DC中的动态表达具有不同的调控机制,这种动态变化参与了着床过程中局部免疫耐受的形成及调控。
Objective: To study the different expression characteristics and dynamic changes of ICAM1 in peripheral blood lymphocytes (PBLC), endometrium and decidual cells (EC / DC) during implantation. METHODS: The implantation mice were used as animal models. The cells were analyzed by monoclonal antibody immunofluorescence and multi-parameter flow cytometry. Results: The expression of ICAM-1 in PBLC and EC / DC showed significant dynamic changes. Among them, the expression of ICAM-1 reached the lowest level in PBLC on day 2 of pregnancy (D2), while in EC / DC Day 4 of gestation (D4) is minimized. CONCLUSION: The dynamic expression of ICAM-1 in PBLC and EC / DC has different regulatory mechanisms. This dynamic change is involved in the formation and regulation of local immune tolerance during implantation.