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在体外保温的条件下,用胶原酶消化鼠的胰岛,对甘露糖和葡萄糖氧化同时伴有~(4 5)Ca摄取和胰岛素释放的反应剂量进行详细的研究。对于~(1 4)C-U-D-葡萄糖氧化的阈值,葡萄糖刺激~(4 5)Ca摄取及胰岛素的释放阈值大约是5.5mM。糖氧化、~(4 5)Ca摄取、胰岛素释放这三种参数最大值一半时葡萄糖浓度分别是13.4mM、11.6mM和12.2mM,它们的最大反应值约为20mM。对于甘露糖氧化的阈值,使钙摄取和胰岛素释放的阈值大约是11.0mM,最大反应限度一半时甘露糖浓度分别为24.6mM,20.5mM和22.2
In vitro incubation conditions, collagenase digestion of rat islets, mannose and glucose oxidation accompanied by ~ (45) Ca uptake and insulin response dose response were studied in detail. For the threshold of ~ (1 4) C-U-D-glucose oxidation, the glucose-stimulated ~ (45) Ca uptake and insulin release threshold was approximately 5.5 mM. Glucose concentrations were 13.4 mM, 11.6 mM and 12.2 mM, respectively, at the maximum of three parameters of glucose oxidation, ~ (45) Ca uptake and insulin release. Their maximal response was about 20 mM. For the threshold of mannose oxidation, the threshold for calcium uptake and insulin release is approximately 11.0 mM and mannose concentrations are 24.6 mM, 20.5 mM and 22.2, respectively, for half the maximum response limit