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目的测定叶酸片中叶酸含量。方法以高效液相色谱法为主要测定方法,分别配制对照品与供试品溶液,控制色谱条件,展开色谱行为与系统适应性试验、线性关系试验、精密度试验、重复性试验、稳定性试验、回收率试验与样品含量测定试验,观察试验结果,判断叶酸片中的叶酸含量。结果叶酸样品溶液24h内稳定、在20.8~180.72μg/ml范围内,线性关系良好,试验仪器精密度强,重复性良好,回收率平均值100.5%,试验准确度良好,叶酸片标示百分含量为100.5%。结论高效液相色谱法测定叶酸片中叶酸含量稳定性强、准确度高,可将其用于叶酸片叶酸含量的测定中,提高测量结果准确度。
Objective To determine the content of folic acid in folic acid tablets. Methods High performance liquid chromatography (HPLC) as the main method was used to prepare reference substance and test solution, control chromatographic conditions, conduct chromatographic behavior and system suitability test, linear relationship test, precision test, repeatability test and stability test , Recovery test and determination of the content of the sample test to observe the test results to determine the folic acid tablets folic acid content. Results The folic acid sample solution was stable within 24 hours, with a good linearity in the range of 20.8-180.72μg / ml. The precision and repeatability of the test instrument were good, with the average recovery of 100.5%. The test accuracy was good. The folic acid tablets labeled percentage Is 100.5%. Conclusion The determination of folic acid in folic acid tablets by HPLC has strong stability and high accuracy. It can be used in the determination of folic acid in folic acid tablets and improve the accuracy of measurement results.