论文部分内容阅读
白头翁叶斑病是近年来我国新报道发生的一种白头翁叶部病害,在辽宁省保护地白头翁生产中病害尤为严重.对采自辽宁省5个白头翁主产区的25株白头翁叶斑病菌(Ascochyta anemones)菌株及中国农业科学院惠赠的5个壳二孢属(Ascochyta spp.)菌株进行RAPD分析.结果表明:11条随机引物共扩增得到条带清晰并呈多态性的108条谱带,单个引物扩增DNA片段集中分布在200～2000bp.采用NTSYS软件进行病原菌的聚类分析,发现供试的30株壳二孢菌的遗传相似系数为0.56～0.98,当相似系数为0.62时,30个菌株被划分为4个类群,说明辽宁省白头翁叶斑病菌具有丰富的遗传多样性.RAPD遗传聚类组群与白头翁叶斑病菌菌株的地理来源有一定的相关性,不同寄主来源的菌株之间存在明显的遗传差异.
Pulsatilla leaf spot is a newly reported Pulsatilla leaf disease in our country in recent years, especially in the production of Pulsatilla in Liaoning Province.Among 25 Pulsatilla spp. Ascochyta anemones strains and five Ascochyta spp. Strains from the Chinese Academy of Agricultural Sciences were analyzed by RAPD.The results showed that there were 108 bands with clear and polymorphic bands amplified by 11 random primers , A single primer amplified DNA fragments concentrated in the 200 ~ 2000bp. NTSYS software for cluster analysis of pathogens and found that tested 30 Ascochyta genetic similarity coefficient of 0.56 to 0.98, when the similarity coefficient of 0.62, 30 strains were divided into four groups, indicating that Liaoning Province Pulsatilla has rich genetic diversity.RAPD genetic clustering group and the Pulsatilla strain geographic sources have some relevance, different host strains There are obvious genetic differences between.