17-酮类固醇的定量在实验室中尚存在许多问题,最主要的是由于非酮类发色物质的干扰,虽然许多研究者采用了一些方法,但仍有一定限制,或则太费时间。作者根据 Drekter氏方法及 Zimmerman 氏反应而设计本法,即利用氯仿分离酮类有色物质,在氯仿层中滴加聚氧乙烯二醇(P.E.G)破坏乳化。作者用脱氢异雄酮(Dehydroisoandrosterone)作为标准物 Quantitation of 17-keto-steroids There are many problems in the laboratory, mainly due to the interference of non-ketone chromogenic substances. Although many researchers adopt some methods, there are still some limitations or it is too time-consuming. The authors designed this method according to the Drekter’s method and the Zimmerman’s reaction, which uses chloroform to separate the ketone-colored material, and then drops the polyoxyethylene glycol (P.E.G.) in the chloroform layer to destroy the emulsion. The author uses dehydroisoandrosterone as a standard