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目的:观察黄芪散对肝癌荷瘤小鼠P53基因表达的影响。方法:将H22肝癌荷瘤小鼠50只随机分为5组,中药高、中、低剂量组分别予以0.4ml中药灌胃,CTX组予以0.2ml(小鼠给药剂量为20mg/kg.d-)腹腔注射,模型组予以0.4ml生理盐水灌胃,每日1次,给药14天;免疫组织化学法检测肿瘤组织P53基因的表达,同时考察脾、胸腺指数及肿瘤抑制率。结果:黄芪散高中低剂量组都能降低H22肝癌荷瘤小鼠癌组织中P53基因的表达水平,且能升高小鼠的脾、胸腺指数。结论:黄芪散对H22肝癌荷瘤小鼠癌组织中P53基因的表达具有一定抑制作用,且能保护小鼠的免疫器官。
Objective: To observe the effect of Astragalus membranaceus on P53 gene expression in hepatoma tumor-bearing mice. Methods: 50 H22 hepatoma tumor-bearing mice were randomly divided into 5 groups. The rats in high, medium and low dose groups were given 0.4ml Chinese herbal medicine respectively. The mice in CTX group were given 0.2ml dose of 20mg / kg.d -) intraperitoneal injection, the model group was given 0.4ml saline intragastrically, once a day, for 14 days; immunohistochemistry was used to detect the expression of P53 gene in the tumor tissue. Meanwhile, the spleen, thymus index and tumor inhibition rate were examined. Results: Astragalus San high and medium low dose group can reduce the expression of P53 gene in H22 hepatoma tumor-bearing mice, and can increase the spleen and thymus index in mice. Conclusion: Astragalus membranaceus could inhibit the expression of P53 gene in H22 hepatoma tumor-bearing mice and protect the immune organs of mice.