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目的：探讨单纯疱疹病毒Ⅰ型胸苷激酶基因（ＴＫ）逆转录病毒载体生产细胞（ｐＬＴＫｃＳＮ／ＶＰＣ）和更昔洛韦（ＧＣＶ）系统杀伤恶性胶质瘤细胞过程中的旁观者效应。方法：采用大鼠胶质瘤细胞Ｃ６、人恶性胶质瘤Ｕ８７ＭＧ和它们的转染ＴＫ阳性细胞Ｃ６ＴＫ，Ｕ８７ＴＫ，将Ｃ６与Ｃ６ＴＫ，Ｕ８７ＭＧ和Ｕ８７ＴＫ按比例（ＴＫ阳性细胞占细胞总体的０％～１００％，１０％梯度）分别混合培养于９６孔板中，每种混合比例设６孔。培养２４ｈ后，６孔中３孔加入浓度为０．５μｇ／ｍｌＧＣＶ，另外３孔作为空白对照。继续培养７２ｈ，直接计数各孔活细胞数并以对照组计数结果为本底计算ＧＣＶ对各混合比例的抑制率。结果：当ＴＫ阳性细胞占１０％时，Ｃ６和Ｕ８７ＭＧ组的抑制率达到或超过３０％，当ＴＫ阳性细胞占总体的５０％以上时，ＧＣＶ对各混合比例的抑制率接近１００％。结论：ｐＬＴＫｃＳＮ／ＶＰＣ和ＧＣＶ系统杀伤恶性胶质瘤细胞过程中存在明显的旁观者效应。
Objective: To investigate the bystander effect of herpes simplex virus type 1 thymidine kinase gene (TK) retroviral vector-producing cells (pLTKcSN/VPC) and ganciclovir (GCV) system in killing malignant glioma cells. METHODS: Rat glioma cells C6, human glioblastoma U87MG and their transfected TK positive cells C6TK, U87TK were used, and C6 was proportional to C6TK, U87MG and U87TK (TK positive cells accounted for 0% of the total cell population. 100%, 10% gradient) were separately mixed and cultured in 96-well plates with 6 wells for each mixing ratio. After 24 hours culture, 3 wells of 6 wells were added with a concentration of 0.5 μg/ml GCV, and 3 wells were used as blank control. After 72 hours of continuous incubation, the number of viable cells in each well was directly counted, and the inhibitory rate of GCV to each mixture ratio was calculated based on the results of the control group. RESULTS: When TK-positive cells accounted for 10%, the inhibition rate of C6 and U87MG groups reached or exceeded 30%. When TK-positive cells accounted for more than 50% of the total, the inhibitory rate of GCV to each mixture ratio was close to 100%. CONCLUSIONS: There are significant bystander effects in the killing of malignant glioma cells by pLTKcSN/VPC and GCV systems.