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目的探讨人脑膜瘤裸鼠皮下移植模型的建立方法。方法将手术切除的4例新鲜人脑膜瘤标本制成组织块,接种于15只裸鼠的皮下,连续喂养12周,观察各组皮下肿瘤的生长情况和组织形态特征,并进行移植瘤传代实验和染色体鉴定。结果接种的15只裸鼠中12只有皮下肿瘤生长,成瘤率80％;皮下移植瘤无明显浸润生长,由裸鼠皮下血管供血,其病理形态特征与人脑膜瘤基本一致;传代后组织学类型与细胞核型均未改变。结论裸鼠皮下移植是建立人脑膜瘤动物模型的可靠方法,可用于脑膜瘤的基础和临床研究。
Objective To investigate the method of establishing subcutaneous human meningioma model in nude mice. Methods Four fresh human meningioma specimens were excised and subcutaneously inoculated into 15 nude mice for 12 weeks. The growth and histomorphology of subcutaneous tumors in each group were observed and the xenograft tumors were passaged And chromosome identification. Results Twelve of the 15 inoculated nude mice were subcutaneously tumor-bearing, with a tumorigenic rate of 80%. Subcutaneous xenografts showed no significant infiltration and growth, and were subcutaneously transplanted into the nude mice. The pathological features were basically the same as those of human meningioma. Neither type nor cell karyotype changed. Conclusion Subcutaneous transplantation of nude mice is a reliable method to establish animal models of human meningioma and can be used in basic and clinical studies of meningiomas.