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精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义.以大斑病的感病自交系“获白”为母本,抗病自交系“77Ht2”为父本构建了F2作图群体.通过RFLP分析知,Ht2基因定位在第8染色体的UMC89和BNL2.369之间,与BNL2.369相距0.9 cM.然后选用在该区域的SSR标记进行了连锁分析,确定了SSR标记UMC1202BNLG1152,UMC1149与Ht2基因紧密连锁,其中UMC1149与Ht2基因的距离为7.2 cM.利用BSA法从450个RAPD引物的扩增产物中筛选出7个可能与Ht2基因连锁的标记,并将其中单拷贝标记转换为SCAR标记.连锁分析表明,一个SCAR标记(定名为SD-06633)距Ht2基因0.4 cM.在此基础上,构建了Ht2基因所在区域的分子标记连锁图.
It is of great significance to fine mapping the locus tumefaciens resistance gene Ht2 to map the gene and explore the distribution of disease resistance genes and molecular marker-assisted selection in maize genome. The F2 mapping population was constructed for the female parent and resistant inbred line “77Ht2”, and the RFLP analysis showed that the Ht2 gene was mapped on the 8th chromosome between UMC89 and BNL2.369, 0.9 cM from BNL2.369 Then the SSR markers in this region were used for linkage analysis, and the SSR markers UMC1202BNLG1152 and UMC1149 were closely linked to Ht2 gene, and the distance between UMC1149 and Ht2 gene was 7.2 cM.The amplification products of 450 RAPD primers We screened out 7 possible markers linked to Ht2 gene and converted the single copy marker into SCAR marker.The linkage analysis showed that a SCAR marker named SD-06633 was 0.4 cM from Ht2 gene.On this basis, Ht2 gene region where the molecular marker linkage map.