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目的：探讨硒在机体自然抗肿瘤发生过程中的作用。方法：用 ＭＮＮＧ（２０ ｍｇ/ｋｇ）灌胃诱导大鼠腺胃粘膜异倍体形成（大鼠腺胃癌模型形成）。用流式细胞仪测定硒对大鼠腺胃幽门粘膜异倍体形成的影响，用免疫组织化学ＡＢＣ法观察硒在大鼠腺胃粘膜异倍体形成过程中，大鼠腺胃、胰ＣＧＲＰ阳性细胞的变化。并对以上结果进行图像分析及统计学处理。结果：（１）硒能抑制ＭＮＮＧ所致大鼠腺胃粘膜细胞异倍体的形成。（２）在ＭＮＮＧ所致大鼠异倍体的形成过程中，其腺胃、胰ＣＧＲＰ阳性细胞的免疫组织化学反应与正常组大鼠相比较均增强（Ｐ＜０．０５，Ｐ＜０。０１）；而在加硒饲料组大鼠腺胃、胰中 ＣＧＲＰ阳性细胞的免疫组织化学反应与正常组大鼠相比较无显著性差异，但与实验对照组大鼠相比较则分别减弱（Ｐ＞０．０５）和明显减弱（Ｐ＜０．０１）。结论：硒和ＣＧＲＰ阳性细胞可能参与了预防和抑制ＭＮＮＧ所致胃粘膜细胞异倍体的形成。
Objective: To explore the role of selenium in the natural anti-tumor process of the body. METHODS: Rats were treated with MNNG (20 mg/kg) to induce gastric mucosal aneuploidy (rat gastric adenocarcinoma model). Flow cytometry was used to determine the effect of selenium on aneuploid formation of rat pyloric mucosa. Immunohistochemical ABC method was used to observe the selenium in the adeno-gastric aneuploid formation in rats. Changes in the cells. The above results were analyzed by image analysis and statistical analysis. RESULTS: (1) Selenium could inhibit the formation of aneuploid cells in rat gastric adeno-gastric mucosa cells induced by MNNG. (2) In the process of MNNG-induced rat aneuploidy, the immunohistochemical reaction of glandular stomach and pancreatic CGRP-positive cells was enhanced compared with normal rats (P<0.05, P<0. 01); In the selenium-enriched diet, the immunohistochemical reaction of CGRP-positive cells in the glandular stomach and pancreas was not significantly different from that in the normal group, but it was decreased compared with the experimental control group (P). >0.05) and significantly decreased (P<0.01). CONCLUSION: Selenium and CGRP positive cells may be involved in the prevention and inhibition of MNNG-induced aneuploidy in gastric mucosal cells.