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为了研究大豆异黄酮活性成分genistein对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)制备的去卵巢(OVX)Parkinson病(PD)模型小鼠黑质纹状体通路的保护作用,我们将OVX PD小鼠随机分为对照组、MPTP组、genistein预处理组和雌激素预处理组,采用免疫组织化学染色结合逆转录-聚合酶链式反应法(RT-PCR)检测黑质致密带(SNpc)神经元酪氨酸羟化酶(TH)的表达情况,采用高效液相色谱法(HPLC-ECD)检测小鼠纹状体(Str)多巴胺(DA)及其代谢物二羟基苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果显示:Genistein及雌激素用药组SN内TH阳性神经元数量及THmRNA的表达水平较MPTP组显著升高(P<0.01)。MPTP组Str内DA及其代谢产物DOPAC和HVA的含量较对照组明显降低(P<0.01)。genistein及雌激素用药组Str内DA、DOPAC及HVA含量较MPTP组显著升高(P<0.01)。上述结果提示genistein对MPTP制备的PD模型小鼠黑质DA能神经元有明显的保护作用。
In order to investigate the genistein activity of genistein against nigusts (OVX) Parkinson disease (PD) model mice prepared from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) The protective effect of striatum pathway. We randomly divided OVX PD mice into control group, MPTP group, genistein pretreatment group and estrogen pretreatment group. Immunohistochemical staining and reverse transcription-polymerase chain reaction RT-PCR was used to detect the tyrosine hydroxylase (TH) expression in substantia nigra pars compacta (SNpc) neurons. Striatal dopamine (DA) was detected by high performance liquid chromatography (HPLC- ) And its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). The results showed that the number of TH-positive neurons and the expression of TH mRNA in genistein and estrogen-treated SN group were significantly higher than those in MPTP group (P <0.01). The contents of DA and its metabolites DOPAC and HVA in Str were lower in MPTP group than those in control group (P <0.01). The contents of DA, DOPAC and HVA in genistein and estrogen treatment groups were significantly higher than those in MPTP group (P <0.01). The above results suggest that genistein has obvious protective effect on dopaminergic neurons in substantia nigra of PD model mice induced by MPTP.