论文部分内容阅读
目的用反相高效液相色谱法测定辣椒风湿巴布膏(辣椒、薄荷脑、冰片)中辣椒素和二氢辣椒素。方法辣椒风湿巴布膏用甲醇萃取,萃取物的HPLC分析采用Hypersil C18分析柱(200 mm×4.6 mm);流动相乙腈-0.1%磷酸水溶液(50:50),检测波长280 nm;体积流量1 mL/min;柱温25℃。结果辣椒素和二氢辣椒素分别在2.443～24.43μg/mL(r=0.999 6)和1.331～13.31μg/mL(r=0.999 8)质量浓度范围内呈线性。辣椒素和二氢辣椒素加样回收率分别为100.9%(RSD为1.1%)和101.1%(RSD为0.6%)。结论实验简便,可靠,对提高药品质量标准提供一个参考方法。
Objective To determine capsaicin and capsaicin in pepper rheumatism cream (pepper, menthol, borneol) by RP-HPLC. Methods Pepper’s rheumatism cream was extracted with methanol. The HPLC analysis of the extract was carried out on a Hypersil C18 column (200 mm × 4.6 mm). The mobile phase was acetonitrile-0.1% phosphoric acid solution (50:50) and the detection wavelength was set at 280 nm. mL / min; column temperature 25 ℃. Results Capsaicin and capsaicin were linear in the mass concentration range of 2.443 ~ 24.43μg / mL (r = 0.999 6) and 1.331 ~ 13.31μg / mL (r = 0.999 8) respectively. The recoveries for capsaicin and capsaicin were 100.9% (RSD 1.1%) and 101.1% (RSD 0.6%), respectively. Conclusion The experiment is simple and reliable, providing a reference method for improving the quality standard of medicines.