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蛋白质组学利用一系列的技术,在基因组规模分析蛋白质表达情况,主要包括蛋白质表达丰度及其翻译后修饰等,从而分析组织或细胞的所有蛋白质的表达、结构和功能,最终实现临床应用。其主要技术系统包括:双向电泳、图像分析系统、蛋白鉴定方法、高通量系统与大规模处理机器人、数据库设置与检索系统。目前最常用的技术路线是蛋白质化学分离、二维电泳(2-DE)与蛋白质谱鉴定。该文介绍蛋白质组学及其研究技术,并对蛋白质组学在系统性红斑狼疮(SLE)基础研究中的应用进行综述。
Proteomics uses a series of techniques to analyze the expression of proteins at genome-wide scale, including the abundance of proteins and their post-translational modifications, to analyze the expression, structure and function of all proteins in tissues and cells, and ultimately achieve clinical application. Its main technical systems include: two-dimensional electrophoresis, image analysis systems, protein identification methods, high-throughput systems and large-scale processing robots, database settings and retrieval system. At present, the most common technical route is protein chemical separation, two-dimensional electrophoresis (2-DE) and protein spectrum identification. This article introduces proteomics and its research techniques, and reviews the application of proteomics in the basic research of systemic lupus erythematosus (SLE).