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为了观察细胞松弛素D(CD)对人类精子顶体反应(AR)的诱导作用,分别使用不同浓度的CD处理获能后的人精子,以钙离子载体A23187作为阳性对照,采用双重染色法检测精子的AR发生率。结果当CD浓度为30μmol/L及500μmol/L时,人精子的AR发生率分别为28.0±9.0%及28.6±2.8%,与空白对照组的17.9±5.5%相比较有明显增加,差异有显著性(P<0.05)。表明适当浓度的CD是一种良好的人类精子顶体反应诱导剂
In order to observe the inducing effect of cytochalasin D (CD) on human sperm acrosome reaction (AR), different concentrations of CD were used to treat the harvested human sperm, calcium ionophore A23187 was used as a positive control and double staining Sperm AR incidence. Results When the concentration of CD was 30μmol / L and 500μmol / L, the incidence of AR in human sperm was 28.0 ± 9.0% and 28.6 ± 2.8% respectively, which was significantly lower than that of the control group .5% compared with a significant increase, the difference was significant (P <0.05). CD showed that the appropriate concentration is a good human sperm acrosome reaction inducer