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为了观察细胞松弛素Ｄ（ＣＤ）对人类精子顶体反应（ＡＲ）的诱导作用，分别使用不同浓度的ＣＤ处理获能后的人精子，以钙离子载体Ａ２３１８７作为阳性对照，采用双重染色法检测精子的ＡＲ发生率。结果当ＣＤ浓度为３０μｍｏｌ／Ｌ及５００μｍｏｌ／Ｌ时，人精子的ＡＲ发生率分别为２８．０±９．０％及２８．６±２．８％，与空白对照组的１７．９±５．５％相比较有明显增加，差异有显著性（Ｐ＜０．０５）。表明适当浓度的ＣＤ是一种良好的人类精子顶体反应诱导剂
In order to observe the inducing effect of cytochalasin D (CD) on human sperm acrosome reaction (AR), different concentrations of CD were used to treat the harvested human sperm, calcium ionophore A23187 was used as a positive control and double staining Sperm AR incidence. Results When the concentration of CD was 30μmol / L and 500μmol / L, the incidence of AR in human sperm was 28.0 ± 9.0% and 28.6 ± 2.8% respectively, which was significantly lower than that of the control group .5% compared with a significant increase, the difference was significant (P <0.05). CD showed that the appropriate concentration is a good human sperm acrosome reaction inducer