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目的探讨运动与白藜芦醇对老年肥胖大鼠内脏脂肪组织视黄醇结合蛋白4(RBP4)mRNA和蛋白表达、血浆RBP4浓度、血糖及胰岛素敏感性的影响。方法建立SD老年肥胖大鼠模型,选取自然生长老年大鼠6只为正常组,将24只肥胖鼠随机分为肥胖对照、运动、白藜芦醇、运动+白藜芦醇4组。白藜芦醇为52.5 mg/(kg·d)灌胃,5次/周,持续干预8周。干预后测血糖、胰岛素敏感性、内脏脂肪组织RBP4 mRNA表达(qRT-PCR)、蛋白表达(Western blot)及血浆RBP4浓度(ELISA)。结果 8周干预后,(1)RBP4 mRNA表达:肥胖组(2.63±0.45)高于正常组(2.10±0.15)(P<0.05);白藜芦醇组(1.84±0.33)、运动组(1.91±0.15)和运动+白藜芦醇组(2.13±0.11)低于肥胖组(P<0.05,P<0.01)。(2)RBP4蛋白表达:肥胖组(1.346±0.025)高于正常组(1.196±0.017)(P<0.01);运动组(1.025±0.006)低于肥胖组(P<0.01)、白藜芦醇组(0.735±0.015)和运动+白藜芦醇组(0.701±0.018)低于运动组(P<0.01);运动+白藜芦醇组低于白藜芦醇组(P<0.05)。(3)血浆RBP4浓度:肥胖组[(16.00±1.54)μg/L]高于正常组[(13.02±2.20)μg/L](P<0.01);运动组[(14.76±1.56)μg/L]低于肥胖组,但差异无统计学意义;白藜芦醇组[(13.59±0.07)μg/L]和运动+白藜芦醇组[(12.98±1.69)μg/L]低于肥胖组(P<0.05,P<0.01)。(4)血糖:肥胖组[(17.93±6.09)mmol/L]高于正常组[(11.64±3.57)mmol/L](P<0.01);运动组[(13.36±1.82)mmol/L]低于肥胖组(P<0.05),白藜芦醇组[(15.24±2.19)mmol/L]、运动+白藜芦醇组[(13.95±2.26)mmol/L]低于肥胖组,但差异无统计学意义。(5)胰岛素敏感性:肥胖组(0.37±0.02)低于正常组(0.39±0.02)(P<0.05);白藜芦醇组(0.38±0.01)、运动组(0.38±0.02)高于肥胖组,但差异无统计学意义,运动+白藜芦醇组(0.39±0.15)高于肥胖组(P<0.05)。上述指标(除蛋白表达外)干预各组间差异无统计学意义。结论老年肥胖大鼠内脏脂肪组织RBP4 mRNA、蛋白表达、血浆RBP4浓度及血糖均增高,胰岛素敏感性降低;运动与白藜芦醇均能降低其RBP4 mRNA、蛋白表达和血浆浓度;单纯运动能明显降低其血糖,运动结合白藜芦醇能明显提高其胰岛素敏感性。在降低蛋白表达、血浆RBP4浓度和提高胰岛素敏感性中,运动结合白藜芦醇表现出协同性。
Objective To investigate the effects of exercise and resveratrol on visceral adipose tissue protein and protein expression of RBP4, plasma RBP4, blood glucose and insulin sensitivity in aged obese rats. Methods SD old obese rat model was established. Six of the aged rats were randomly divided into four groups: obese control, exercise, resveratrol, exercise + resveratrol. Resveratrol was intragastrically administered at 52.5 mg / (kg · d) for 5 times a week for 8 weeks. Blood glucose, insulin sensitivity, qRT-PCR, Western blot and RBP4 concentration (ELISA) were measured after intervention. Results After intervention for 8 weeks, (1) RBP4 mRNA expression in obese group (2.63 ± 0.45) was significantly higher than that in normal group (2.10 ± 0.15) (P <0.05); resveratrol group (1.84 ± 0.33) ± 0.15) and exercise + resveratrol group (2.13 ± 0.11) than in obese group (P <0.05, P <0.01). (2) The expression of RBP4 protein in obesity group (1.346 ± 0.025) was significantly higher than that in normal group (1.196 ± 0.017) (P <0.01); exercise group (1.025 ± 0.006) was lower than that in obesity group (0.735 ± 0.015) and exercise + resveratrol group (0.701 ± 0.018) were lower than those in exercise group (P <0.01). Exercise + resveratrol group was lower than resveratrol group (P <0.05). (3) Plasma RBP4 concentration: (16.00 ± 1.54) μg / L in obesity group was significantly higher than that in normal group (13.02 ± 2.20) μg / L and 14.76 ± 1.56 μg / L ] Was lower than that of the obese group, but the difference was not statistically significant; resveratrol group ([(13.59 ± 0.07) μg / L] and exercise + resveratrol group [(12.98 ± 1.69) μg / L] (P <0.05, P <0.01). (4) Blood glucose: [(17.93 ± 6.09) mmol / L] in obesity group was significantly higher than that in normal group [(11.64 ± 3.57) mmol / L] (15.24 ± 2.19) mmol / L in resveratrol group and [(13.95 ± 2.26) mmol / L in exercise + resveratrol group) were lower than those in obese group (P <0.05) Statistical significance. (5) Insulin sensitivity was lower in obesity group (0.37 ± 0.02) than in normal group (0.39 ± 0.02) (P <0.05); resveratrol group (0.38 ± 0.01) and exercise group (0.38 ± 0.02) Group, but the difference was not statistically significant. Exercise + resveratrol group (0.39 ± 0.15) was higher than that of obese group (P <0.05). The above indicators (except protein expression) were no significant difference between the intervention groups. CONCLUSIONS: RBP4 mRNA and protein expression, RBP4 concentration and plasma glucose in visceral adipose tissue were increased and insulin sensitivity was decreased in aged obese rats. Both exercise and resveratrol decreased RBP4 mRNA and protein expression and plasma concentration. Reduce their blood sugar, exercise combined with resveratrol can significantly improve its insulin sensitivity. Exercise-bound resveratrol shows synergy in reducing protein expression, plasma RBP4 concentration and increasing insulin sensitivity.