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目的:研究拟胆碱药卡巴胆碱对烫伤大鼠口服补液时小肠TNF-α及水通道蛋白-1(AQP-1)表达的影响.方法:♂Wistar大鼠50只,随机分为假烫(N)、单纯烫伤(S)、肠内葡萄糖-电解质液(GES)、肠内卡巴胆碱组(CAR)和肠内葡萄糖-电解质液+卡巴胆碱组(GES/CAR)5组(n=10).大鼠背部用沸水造成35%TBSA烫伤.N,GES和GES/CAR组于伤后30min开始补液.免疫组化法测定肠组织AQP-1的表达,ELISA法检测肠组织TNF-α含量,酚红法测定大鼠小肠对水的吸收率.结果:S组大鼠小肠AQP-1与N组比明显降低(90.3±18.4vs4851.6±654.5,P<0.01);CAR,GES和GES/CAR组AQP-1与S组相比均显著增加(1806.1±110.1,2272.3±113.8,3322.0±595.9vs90.3±18.4,均P<0.01).给予卡巴胆碱组(CAR,GES/CAR)与未给予卡巴胆碱组(S,GES)相比肠组织TNF-α含量明显下降(0.9±0.3,1.0±0.47vs1.8±0.3,1.9±0.2,P<0.05).GES/CAR,CAR及S组AQP-1表达量与TNF-α含量成负相关(r=-0.9030,-0.9602,-0.9866,均P<0.05).GES/CAR组水吸收率较GES组明显升高(21.0%±0.1%vs12.7%±0.1%,P<0.05).结论:卡巴胆碱可抑制促炎因子TNF-α的释放,上调小肠AQP-1表达,改善大鼠烫伤早期肠道对水的吸收.
Objective: To study the effect of choline choline on the expression of TNF-α and aquaporin-1 (AQP-1) in the intestine of oral liquid in scalded rats.Methods: Fifty male Wistar rats were randomly divided into four groups: (N), scald (S), GES, CAR and GES / CAR groups (n = 10) .The rats were subjected to 35% TBSA scald with boiling water in the back.N, GES and GES / CAR groups were reperfused 30 min after injury.The expression of AQP-1 in intestinal tissue was detected by immunohistochemical method.The expression of TNF- α, the absorption rate of water in the small intestine of rats was measured by the phenol red method.Results: Compared with the N group, the AQP-1 and N of the small intestine in the S group were significantly decreased (90.3 ± 18.4 vs 4851.6 ± 654.5, P <0.01) And GES / CAR group AQP-1 significantly increased compared with S group (1806.1 ± 110.1,2272.3 ± 113.8,3322.0 ± 595.9 vs90.3 ± 18.4, both P <0.01) CAR) significantly decreased the levels of TNF-α in intestinal tissue compared with those without carbachol (S, GES) (0.9 ± 0.3, 1.0 ± 0.47 vs 1.8 ± 0.3, 1.9 ± 0.2, P <0.05) The expression of AQP-1 in CAR and S group was negatively correlated with the level of TNF-α (r = -0.9030, -0.9602, -0.9866, all P <0.05) (21.0% ± 0.1% vs12.7% ± 0.1%, P <0.05) .Conclusion: Carbachol can inhibit the release of proinflammatory cytokine TNF-αand up-regulate the expression of AQP-1 in the small intestine, Improve early intestinal absorption of water in rats with scald.