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目的探讨人脑胶质瘤与猴病毒40SV40的关系及其p53和RB蛋白的表达。方法采用核酸原位杂交和免疫组织化学技术对256例人脑胶质瘤和11例正常脑组织进行了SV40 DNA和抑癌基因产物p53、RB蛋白检测。结果胶质瘤SV40阳性104例，阳性率40．6％，正常脑组织均未检测出SV40 DNA，未发现SV40感染与胶质瘤病理分级存在明显相关P ＞0．05；SV40阳性胶质瘤p53、RB蛋白表达阳性率显著高于SV40阴性胶质瘤P＜ 0．01；p53、RB蛋白表达阳性率分别与胶质瘤病理分级呈正相关Pearson列联系数分别为P＝0．45和P＝0．49 P＜ 0．01。结论我国人脑胶质瘤组织中存在着SV40感染现象；p53、RB蛋白过表达以及SV40感染可能在胶质瘤发生发展中起重要作用，其内在联系有待研究。
Objective To investigate the relationship between human glioma and simian virus 40 SV40 and its p53 and RB protein expression. Methods SV DNA and in situ hybridization and immunohistochemistry were used to detect the expression of p53 and RB proteins of SV40 DNA and tumor suppressor gene in 256 human gliomas and 11 normal brain tissues. Results 104 glioma SV40-positive in 104 cases, the positive rate of 40.6%, normal brain tissue were not detected SV40 DNA, found no significant correlation between SV40 infection and glioma pathological grade P> 0.05 ; SV40 positive The positive rates of p53 and RB proteins in gliomas were significantly higher than those in SV40-negative gliomas (P <0.01). The positive rates of p53 and RB proteins were positively correlated with the pathological grades of gliomas (P < = 0.45 and P = 0.49 P <0.01 . CONCLUSIONS: SV40 infection exists in human glioma tissues in China. Overexpression of p53 and RB proteins and SV40 infection may play an important role in the development of gliomas.