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目的:建立2种藤黄酸异构体的高效液相含量测定方法。方法:采用色谱柱SunFire(Waters)C8(2.1 mm×150mm,3.5μm),流动相为乙腈-甲醇-0.3%三氟乙酸溶液(36∶37∶27),检测波长360 nm,流速0.3 mL·min-1,柱温28℃。结果:R-藤黄酸,S-藤黄酸线性关系方程分别为Y=2.87×106X-2.24×105,r=0.999 9;Y=3.31×106X-1.44×105,r=0.999 9。平均回收率分别为100.0%,100.9%;RSD分别为2.1%,2.5%(n=6)。藤黄药材供试品中R-藤黄酸和S-藤黄酸的平均质量分数为30.06%,21.45%。结论:本方法简便、稳定,可用于藤黄中藤黄酸2种异构体的鉴定与含量测定。
Objective: To establish a method for the determination of two kinds of isomers of gambogic acid. Methods: The chromatographic column SunFire (Waters) C8 (2.1 mm × 150 mm, 3.5 μm) was used. The mobile phase was acetonitrile-methanol-0.3% trifluoroacetic acid solution (36:37:27) min-1, column temperature 28 ℃. Results: The linear equations of R-Gambogic acid and S-Gambogic acid were Y = 2.87 × 106X-2.24 × 105, r = 0.999 9, Y = 3.31 × 106X-1.44 × 105, r = 0.999 9. The average recoveries were 100.0% and 100.9%, respectively. The RSDs were 2.1% and 2.5%, respectively (n = 6). The average mass fraction of R-gambogic acid and S-gambogic acid in the sample was 30.06% and 21.45%, respectively. Conclusion: The method is simple and stable and can be used for the identification and determination of two isomers of gambogic acid in Garcinia Cambogia.