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目的:研究国内主产区忍冬种质资源的遗传多样性,探讨它们之间的亲缘关系,并为新品种选育提供参考。方法:选用6对引物组合对13个忍冬种质进行AFLP分析,利用DPS V3.01软件计算各品种的遗传距离,按UPGMA法进行聚类分析。结果:共获得435条扩增带,其中191条具有多态性,平均多态检出率为43.9%。聚类结果表明,同一道地产区的栽培品种亲缘关系较近,山东种质基于AFLP标记的分类结果与形态特征基本一致。结论:AFLP分子标记揭示出忍冬种内存在丰富的遗传多样性,为忍冬种质资源的合理利用及品种选育提供了分子水平的理论依据。
OBJECTIVE: To study the genetic diversity of honeysuckle germplasm resources in the main producing areas of China, to explore the genetic relationship between them and to provide references for the breeding of new varieties. Methods: AFLP analysis of thirteen honeysuckle germplasms was conducted using 6 pairs of primer combinations. The genetic distance of each species was calculated by DPS V3.01 software, and the cluster analysis was performed according to UPGMA method. RESULTS: A total of 435 amplification bands were obtained, of which 191 were polymorphic and the average detection rate was 43.9%. The clustering results showed that the cultivars in the same real estate area had a close genetic relationship, and the classification results of Shandong germplasm based on AFLP markers were basically consistent with the morphological characteristics. CONCLUSION: The AFLP molecular marker revealed rich genetic diversity within the honeysuckle species, which provided a theoretical basis for the rational utilization of honeysuckle germplasm resources and breed breeding.