论文部分内容阅读
目的观察坏死性小肠结肠炎(NEC)新生大鼠肠道组织含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase 3)与增殖细胞核抗原(PCNA)的表达情况,并探讨谷氨酰胺(Gln)对NEC大鼠肠道组织的保护作用。方法 36只新生SD大鼠随机分为对照组、NEC模型组和Gln+NEC模型组,每组12只。对照组予鼠乳代乳品人工喂养3天;NEC模型组采用鼠乳代乳品喂养+缺氧+冷刺激,连续3天建立NEC模型;Gln+NEC模型组建立NEC模型的同时,给予鼠乳代乳品加Gln喂养。各组大鼠均在3天后处死,HE染色后对其回肠进行病理评分,采用免疫组织化学方法检测肠组织Caspase 3和PCNA的表达情况。结果 NEC模型组肠组织Caspase 3表达指数为(77.3±8.6)%,高于对照组及Gln+NEC模型组[(18.9±3.4)%、(50.3±6.2)%],Gln+NEC模型组高于对照组,差异有统计学意义(P<0.05)。NEC模型组肠组织PCNA表达指数为(15.0±1.9)%,低于对照组及Gln+NEC模型组[(34.2±5.8)%、(24.0±3.9)%],Gln+NEC模型组低于对照组,差异有统计学意义(P<0.05)。NEC模型组肠道组织病理评分与Caspase 3表达指数(r=0.769,P=0.005)、Caspase 3/PCNA表达指数比(r=0.835,P=0.002)成正相关,与PCNA表达指数成负相关(r=-0.698,P=0.014)。结论 Caspase 3可能通过表达上调、PCNA通过表达下调参与NEC的疾病过程,细胞增殖和凋亡失衡与NEC的发生发展有一定关系。Gln在一定范围内抑制细胞凋亡、促进细胞增殖,可能是治疗NEC的有效措施之一。
Objective To observe the expression of caspase 3 and proliferating cell nuclear antigen (PCNA) in intestinal tissue of neonatal rats with necrotizing enterocolitis (NEC) and to investigate the effect of glutamine (Gln) on Intestinal Tissue of NEC Rats. Methods Thirty-six neonatal SD rats were randomly divided into control group, NEC model group and Gln + NEC model group, with 12 rats in each group. The NEC model group was fed with milk milk substitutes + hypoxia + cold stimulation for 3 consecutive days to establish NEC model. While NEC model was established in Gn + NEC model group, mouse milk generation Dairy plus Gln feeding. The rats in each group were sacrificed after 3 days. The pathological scores of the ileum were evaluated by HE staining. The expressions of Caspase 3 and PCNA were detected by immunohistochemistry. Results The expression of Caspase 3 in NEC model group was (77.3 ± 8.6)%, higher than that in control group and Gln + NEC model group [(18.9 ± 3.4)%, (50.3 ± 6.2)%] In the control group, the difference was statistically significant (P <0.05). The expression of PCNA in the NEC model group was (15.0 ± 1.9)% lower than that in the control group and Gln + NEC model group [(34.2 ± 5.8)%, (24.0 ± 3.9)%] Group, the difference was statistically significant (P <0.05). The intestinal histopathological score of NEC model was positively correlated with Caspase 3 expression index (r = 0.769, P = 0.005) and Caspase 3 / PCNA expression index (r = 0.835, P = 0.002) r = -0.698, P = 0.014). Conclusions Caspase 3 may be up-regulated through expression, and PCNA may be involved in the disease process of NEC through down-regulation. The imbalance of cell proliferation and apoptosis has some relationship with the occurrence and development of NEC. Gln in a certain range of inhibition of apoptosis and promote cell proliferation may be one of the effective measures for the treatment of NEC.