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目的:建立呼吸道合胞病毒耳部感染的动物模型。方法:杂色豚鼠15只,按取材时间分为第7、18、25天3组。将含病毒溶液滴入豚鼠双鼻孔,让其自然吸入。按不同时间将豚鼠断头,连同鼻咽和颅底的双侧听泡一起取出,石腊包埋,连续切片,苏木精伊红染色,光镜下观察。结果:第7天有8耳鼓膜充血。咽鼓管腔内有大量中性粒细胞、淋巴细胞、红细胞及纤维素。纤毛上皮细胞退行性变性,纤毛大片脱落,上皮细胞坏死脱落,基底膜暴露。与咽鼓管邻近的听泡上皮高度肿胀,表面见大量炎性细胞。第18天部分上皮细胞变为立方上皮,呈结节状,造成管腔闭塞。1耳耳蜗鼓阶积血,前庭区之膜迷路内有炎性细胞渗出。第25天仅1耳咽鼓管内少量上皮脱落,细胞浸润明显减少。结论:呼吸道合胞病毒鼻腔接种后可引起咽鼓管急性非特异性炎症改变,并蔓延到鼓室,个别严重者可引起内耳炎性反应。
Objective: To establish an animal model of ear infections of respiratory syncytial virus. Methods: Fifteen variegated guinea pigs were divided into 3 groups on the 7th, 18th and 25th days according to the time of drawing. The virus-containing solution into the double nostrils of guinea pigs, let it naturally inhaled. The guinea pigs were decapitated at different times and taken out together with the bilateral pharyngeal and skull bases. Paraffin embedded, serial sections were stained with hematoxylin and eosin, and observed under light microscope. Results: There was 8 eardrum congestion on the 7th day. Eustachian tube has a large number of neutrophils, lymphocytes, red blood cells and cellulose. Degenerative ciliated epithelial degeneration, large cilia off, epithelial necrosis, basement membrane exposure. Adjacent to the eustachian tube to listen to the height of the bulge epithelium swelling, see a large number of inflammatory cells on the surface. On the 18th day, some epithelial cells became cubic epithelium, nodular, causing lumen occlusion. 1 ear cochlea hemorrhage, vestibular area of the labyrinth of inflammatory cell exudation. On the 25th day only a small amount of epithelial shedding in the ear eustachian tube, cell infiltration significantly reduced. Conclusion: Respiratory syncytial virus nasal inoculation can cause eustachian tube acute nonspecific inflammatory changes, and spread to the tympanic cavity, some severe cases can cause inflammation of the inner ear.