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目的探讨威灵仙多糖对人乳腺癌MCF-7细胞凋亡的影响及其分子机制。方法不同浓度威灵仙多糖作用于MCF-7细胞48h后,荧光显微镜观察人乳腺癌细胞MCF-7细胞凋亡情况,RT-PCR法检测Bcl-2、Fas基因表达量的变化。结果威灵仙多糖能诱导MCF-7细胞凋亡,40mg/L时凋亡率为17.63%,实验组与对照组比较有显著性差异;并且上调Fas和降低Bcl-2表达。结论威灵仙多糖能抑制MCF-7细胞增殖,促进其细胞凋亡,其机制可能与激活Fas、抑制Bcl-2的基因表达有关。
Objective To investigate the effect of Clematis on apoptosis of human breast cancer MCF-7 cells and its molecular mechanism. Methods The apoptosis of human breast cancer cell line MCF-7 was observed by fluorescence microscopy after different concentration of Clematis polysaccharide was treated with MCF-7 for 48 hours. The expression of Bcl-2 and Fas gene was detected by RT-PCR. Results Clematis induces the apoptosis of MCF-7 cells. The apoptotic rate of MCF-7 cells is 40% at 17.63%. There is a significant difference between the experimental group and the control group, and the up-regulation of Fas and the decrease of Bcl-2 expression. Conclusion CTX can inhibit the proliferation of MCF-7 cells and promote the apoptosis of MCF-7 cells. The mechanism may be related to the activation of Fas and the inhibition of Bcl-2 gene expression.