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[目的]研究连翘叶提取物(FSE)对力竭及恢复小鼠肝脏、股四头肌SOD、POD、MDA和血清AST、ALT、AKP活性的影响。[方法]72只ICR小鼠随机均分为安静对照组A、安静FSE组B、力竭运动对照组C、力竭运动FSE组D、力竭恢复对照组E、力竭恢复FSE组F,每组12只,各给药组小鼠每日按1.5g生药/kg体重剂量以FSE灌胃,各对照组小鼠以蒸馏水灌胃,连续20d后,将运动组小鼠置于水中游泳至力竭,记录力竭时间。C组和D组小鼠运动后即刻摘眼球采血备用,取肝脏、股四头肌备用。A组和B组不做运动处理,直接摘眼球采血备用,取肝脏、股四头肌备用。E组和F组小鼠恢复24h后摘眼球采血备用,取肝脏、股四头肌备用。测定小鼠肝脏、股四头肌MDA含量、SOD和POD活性及血清中AST、ALT和AKP活性。[结果]FSE可以提高力竭状态下小鼠肝脏、股四头肌中SOD、POD活性,减少MDA生成,并可显著降低血液中AST、ALT、AKP活性。[结论]FSE对力竭运动机体肝脏和骨骼肌有保护作用,并具有促进机体恢复的作用。
[Objective] To investigate the effects of Forsythia suspensa leaf extract (FSE) on exhaustion and recovery of SOD, POD, MDA, serum AST, ALT and AKP activity in mice liver and quadriceps. [Methods] Seventy ICR mice were randomly divided into three groups: quiet control group A, quiet FSE group B, exhausted exercise control group C, exhausted exercise FSE group D, exhausted recovery control group E, exhaustive recovery FSE group F, 12 mice in each group. The mice in each administration group were intragastrically administered FSE at a dosage of 1.5g crude drug / kg body weight daily, and the mice in each control group were given gavage with distilled water. After 20 consecutive days, the mice in the exercise group were swamped in water until Exhausted, record exhausted time. Groups C and D mice immediately after the eyeball blood taken spare, take the liver, quadriceps spare. A group and B group without exercise treatment, direct eyeball blood reserve, take the liver, quadriceps spare. E group and F group of mice recovered 24h after eyeball blood reserve, take the liver, quadriceps spare. The content of MDA, the activities of SOD and POD and the activities of AST, ALT and AKP in the liver and quadriceps were determined. [Result] FSE could increase the activity of SOD and POD in liver and quadriceps muscle of exhausted rats, decrease the production of MDA, and significantly decrease the activity of AST, ALT and AKP in blood. [Conclusion] FSE has a protective effect on the liver and skeletal muscle of exhaustive exercise and has the effect of promoting the body recovery.