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本文对引进的CTLL_2—IL_2依赖细胞株筛选了适宜生长和增殖条件。在此培养条件下,观察研究了CTLL_2的生长特性,同时用此CTLL_2以MTT比色分析法检测了本室制备的人、大鼠和小鼠等不同来源IL_2的生物活性。实验结果表明,在适宜的条件下,此株CTLL_2对几种来源的IL_2反应都很稳定,用其检测IL_2活性简便、稳定、检测所需时间短、重复性好。为进一步研究IL_2提供稳定的检测系统,并为研究T细胞提供一个良好的培养条件。
In this paper, the suitable CTLL 2 -IL 2 -dependent cell lines were screened for suitable growth and proliferation conditions. Under this culture condition, the growth characteristics of CTLL 2 were observed and studied. The biological activity of IL 2 from different sources such as human, rat and mouse prepared in this chamber was detected by MTT colorimetric assay using this CTLL_2. The experimental results show that under suitable conditions, the strain CTLL 2 is stable to IL 2 from several sources. The detection of IL 2 activity using this strain is simple, stable, and the detection time is short and the repeatability is good. To provide a stable detection system for the further study of IL 2 and to provide a good culture condition for studying T cells.