论文部分内容阅读
在温室条件下以枳〔Poncirus trifoliata(L.)Raf.〕组培苗为试材,研究了接种Glomus versiforme和G.mosseae对其生长、碳水化合物和抗氧化酶的影响.结果表明,接种G.versiforme的组培苗和接种G.mosseae的组培苗分别在第二级侧根和第一级侧根中观察到最高的菌根侵染率、泡囊数、丛枝数和侵入点.两种丛枝菌根真菌都显著提高了茎粗、叶面积、叶片数、根系体积、地上部干重、地下部干重、叶绿素和类胡萝卜素含量.两种丛枝菌根真菌显著促进了叶片和根系可溶性糖以及总的非结构碳水化合物含量.丛枝菌根真菌也提高了叶片和根系中SOD、POD和CAT活性,但显著抑制了叶片和根系中可溶性蛋白含量.G.versiforme对柑橘组培苗生长和碳水化合物的促进效果较好;G.mosseae对组培苗抗氧化酶的促进效果较好.表4参26
The effects of inoculation with Glomus versiforme and G. mosseae on the growth, carbohydrates and antioxidant enzyme activities of Poncirus trifoliata (L.) Raf. Were studied under greenhouse conditions. The results showed that inoculation of G The highest rates of mycorrhizal infection, number of vesicles, number of arbuscular branches, and invasive sites were observed in the tissue culture plantlets of G. versiforme and the tissue culture seedlings inoculated with G. mosseae, respectively, in the second and third lateral roots Arbuscular mycorrhizal fungi significantly increased stem diameter, leaf area, number of leaves, root volume, shoot aboveground dry weight, chlorophyll and carotenoid content of the arbuscular mycorrhizal fungi.The two arbuscular mycorrhizal fungi significantly promoted leaf and Root soluble sugar and total unstructured carbohydrate content.The AM fungi also increased the activity of SOD, POD and CAT in leaves and roots, but significantly inhibited the content of soluble protein in leaves and roots.G.versiforme had no significant effect on the tissue culture of citrus Miao growth and carbohydrate promotion effect is better; G.mosseae on tissue culture antioxidant enzyme promotion effect is better .Table 4 参 参 26