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目的:建立芪参健骨颗粒中黄芪甲苷的含量测定方法。方法:采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)。色谱柱:Zorbax Eclipse XDB-C18(4.6×150mm,5μm);流动相:乙腈∶水=32∶68;流速:1.0mL.min-1;柱温:25℃;漂移管温度:65℃;气体流速:1.8L.min-1。结果:黄芪甲苷在1.001~10.01μg范围内线性关系良好,回归方程为Y=1.634X+1.926(r=0.9995),平均加样回收率为98.58%,RSD=1.5%。结论:该方法简便快速、结果准确可靠、方法重复性好,可用于控制芪参健骨颗粒的质量。
Objective: To establish a method for the determination of astragaloside Ⅳ in Qi-shen Jiangu granules. Methods: HPLC-ELSD was used. Column: Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 5 μm); mobile phase: acetonitrile: water = 32:68; flow rate: 1.0 mL.min-1; Flow rate: 1.8 L · min-1. Results: Astragaloside had a good linearity in the range of 1.001 ~ 10.01 μg with a regression equation of Y = 1.634X + 1.926 (r = 0.9995). The average recovery was 98.58% and the RSD was 1.5%. Conclusion: The method is simple and rapid, accurate and reliable, reproducible method and can be used to control the quality of Qishen Jiangu granules.