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目的通过浒苔多糖粗提物对RAW264.7巨噬细胞体外培养的干预研究,探索对该细胞免疫功能的影响。方法对RAW 264.7巨噬细胞进行培养,用四唑氮蓝(MTT)比色法测定巨噬细胞增殖;ELISA法测定细胞因子分泌量的变化。结果在一定浓度范围和干预时间内,浒苔多糖粗提物促进RAW264.7增殖,且随干预浓度增加,TNF-α和IL-6分泌显著增多。结论浒苔多糖粗提物对巨噬细胞RAW264.7具有免疫调节作用。
OBJECTIVE: To investigate the effects of polysaccharide from Enteromorpha prolifera on the in vitro culture of RAW264.7 macrophages in vitro and to explore its effect on immune function. Methods RAW 264.7 macrophages were cultured and the proliferation of macrophages was determined by MTT assay. The changes of cytokine secretion were measured by ELISA. Results The crude extract of Enteromorpha prolifera increased the proliferation of RAW264.7 in a certain range of concentration and time of intervention, and the secretion of TNF-α and IL-6 increased significantly with the increase of intervention concentration. Conclusion The crude polysaccharide Enteromorpha polysaccharide RAW264.7 macrophage immune regulation.