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目的观察乙烯利对成年及子代雌性Balb/c小鼠体液免疫及细胞免疫的影响。方法成年动物以乙烯利灌胃染毒,剂量为15.9、47.7和143 mg/kg,4周后观察小鼠免疫器官脏器系数、外周血淋巴细胞及免疫功能的变化;成年雌性小鼠合笼后,于孕期第6天(GD6)开始灌胃染毒,至仔鼠断乳,仔鼠6周龄时观察仔鼠免疫器官脏器系数、外周血淋巴细胞及免疫功能的变化。采用LDH法测定自然杀伤(NK)细胞活性,采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能,采用溶血空斑实验来评价体液免疫功能。结果各剂量组成年小鼠及仔鼠体重及脏器系数未发生改变;143 mg/kg乙烯利能够使仔鼠淋巴细胞数增多,而成年小鼠未产生变化;143mg/kg的乙烯利能够抑制成年小鼠的NK细胞活性,但是47.7～143mg/kg的乙烯利却增强仔鼠的NK细胞活性;15.9～143 mg/kg乙烯利能够增强雌性小鼠T淋巴细胞增殖能力,但是子代小鼠各剂量组的T淋巴细胞增殖能力却未受影响;成年小鼠各剂量组迟发型变态反应均未发生显著变化,47.7～143 mg/kg浓度的乙烯利能够抑制仔鼠迟发型变态反应的发生;成年及仔鼠的体液免疫功能未受影响。结论乙烯利在15.9～143 mg/kg时能够影响成年及子代Balb/c小鼠的免疫系统,成年及子代小鼠免疫系统对乙烯利的反应性存在差异,表明同一化学物的免疫毒性及发育免疫毒性表现不完全相同;评价外源化学物对免疫系统的影响时,有必要时同时观察外源化学物对免疫系统及发育免疫系统的影响。
Objective To investigate the effects of ethephon on humoral and cellular immunity in female and offspring female Balb / c mice. Methods Adult animals were treated with ethephon intragastrically at dose of 15.9, 47.7 and 143 mg / kg. After 4 weeks, the organs of immune organs, peripheral blood lymphocytes and immune function were observed. Adult female mice were housed in cage After the first 6 days of gestation (GD6), gavage was started and the weaned piglets were sacrificed. The organs of immune organs, the peripheral blood lymphocytes and the immune function of the offspring were observed at 6 weeks of age. Natural killer (NK) cell activity was determined by LDH assay, cellular immune function was evaluated by T lymphocyte proliferation and delayed-type hypersensitivity, and hemolytic plaque assay was used to evaluate humoral immune function. Results Body weight and organ coefficient of mice and pups did not change in each dose group; 143 mg / kg ethephon could increase the number of lymphocytes in the offspring, but did not change in adult mice; ethaline at 143 mg / kg inhibited However, 47.7 ~ 143mg / kg of ethephon enhanced the NK cell activity of the offspring; 15.9 ~ 143 mg / kg of ethephon enhanced the T lymphocyte proliferation of the female mice, but the offspring mice T lymphocyte proliferation ability of each dose group was not affected; no significant changes were observed in the delayed-type hypersensitivity in each dose group of adult mice. The ethephon concentration of 47.7 ~ 143 mg / kg could inhibit the occurrence of delayed type hypersensitivity The humoral immune function of adult and offspring was unaffected. CONCLUSION Ethephon can affect the immune system of adult and offspring Balb / c mice at 15.9-143 mg / kg. There is a difference in the response to ethephon between the immune system of adult and offspring mice, indicating that the same chemical is immunotoxic And development of immunotoxicity are not exactly the same; evaluation of foreign chemicals on the immune system, when necessary, simultaneously observe the impact of foreign chemicals on the immune system and the development of the immune system.