论文部分内容阅读
目的探讨血管内皮生长因子(VEGF)各种转录本亚型在成骨细胞分化中的作用。方法应用特殊设计的引物,逆转录聚合酶链反应(RTPCR)检测VEGFmRNA在人类成骨细胞样细胞SaOS2和MG63和胎鼠颅盖骨细胞(FRC)在第0、5、9、14天不同分化阶段中的选择性剪接表达。结果人类成骨细胞样细胞SaOS2和MG63表达所有的VEGFmRNA亚型,其中以VEGF121和VEGF165表达最强。鼠FRC细胞表达3种VEGFmRNA亚型(即VEGF120,VEGF144和VEGF164),其中以VEGF120和VEGF164表达最强。伴随着FRC细胞的增殖、分化和矿化,这些亚型的表达逐渐增强,并且在结节矿化后达到最高水平。结论小分子的VEGF121/120和VEGF165/164在成骨细胞的分化过程中表达增强,可能起重要的血管源性调节作用。
Objective To investigate the role of various transcript subtypes of vascular endothelial growth factor (VEGF) in the differentiation of osteoblasts. Methods Specific primers and reverse transcriptase polymerase chain reaction (RTPCR) were used to detect the differential expression of VEGF mRNA in human osteoblast-like cells Saos2 and MG63 and fetal rat calvarial cells (FRC) on days 0, 5, 9 and 14 Selective splicing in stages. Results Human osteoblast-like cells SaOS2 and MG63 expressed all the VEGF mRNA subtypes, of which the most expressed was VEGF121 and VEGF165. Mouse FRC cells express three subtypes of VEGFmRNA (ie, VEGF120, VEGF144 and VEGF164), of which VEGF120 and VEGF164 express the strongest. Along with the proliferation, differentiation and mineralization of FRC cells, the expression of these isoforms gradually increases and reaches the highest level after nodule mineralization. Conclusion The small molecule expression of VEGF121 / 120 and VEGF165 / 164 in osteoblast differentiation may play an important role in the regulation of vasogenicity.