论文部分内容阅读
细胞的DNA非程序合成(简称UDS)作为一种反映DNA修复合成的指标,已广泛应用于研究化学物质和物理因子的细胞遗传毒理作用,并已被认为可用作致癌物的快速筛选。过去,研究者曾采用体外培养的人外周血淋巴细胞、人成纤维细胞、HeLa细胞、大鼠肝原代培养细胞等,成功地显示了由致癌性化合物所诱导的UDS,其中人外周血淋巴细胞的UDS,由于其取材方便,已在国内许多实验室建立。但以往的方法,常用PHA激活淋巴细胞,需时较长,操作较繁琐。本
The DNA non-programmable synthesis (abbreviated as UDS) of cells has been widely used as a marker to reflect the DNA repair and synthesis, and has been widely used to study cytogenetic toxicological effects of chemical substances and physical factors, and has been considered as a rapid screening of carcinogens. In the past, researchers have used in vitro cultured human peripheral blood lymphocytes, human fibroblasts, HeLa cells, rat liver primary culture cells, etc., successfully demonstrated UDS induced by carcinogenic compounds, including human peripheral blood lymphoid The UDS of cells has been established in many laboratories in China because of its convenient materials. However, in the previous methods, PHA frequently used lymphocyte activation, which required a long time and was cumbersome to perform. this