论文部分内容阅读
目的 研究黑龙江省肾综合征出血热(HFRS)患者感染汉坦病毒(HV)的分子特征及其与鼠携带HV的对应关系.方法 从HFRS患者血标本中成功分离到一株HV,编号WCL,对病毒S基因进行扩增和序列分析.结果WCL株S基因全长由1698 nt组成,TA含量丰富,编码N蛋白的ORF起始于37nt,终止于1326 nt,编码的蛋白长429 aa,符合HTN型编码.与HV参考毒株进行比较,WCL与汉滩型(HTN)同源性较高,尤其与黑龙江省黑线姬鼠分离株Bao14同源性最高,与汉城型(SEO)等其他病毒同源性较低,并且WCL的S基因编码的N蛋白氨基酸序列也比较保守.进化树分析表明,WCL位于HTN型病毒所在支系,与Bao14、HTN261处于同一分支并且更接近,同属于H4亚型,体现了一定的地理簇集性.结论 WCL与黑龙江省黑线姬鼠分离株属同一亚型,首次发现有H4亚型病例分布.“,”Objective To study the molecular characterization of a strain of hantavirus isolated from a patient with hemorrhagic fever with renal syndrome ( HFRS) in Heilongjiang province and its corresponding relationship with hantaviruses in rats. Methods A strain of hantavirus was isolated from the serum sample of a HFRS patient, and named WCL. Then the S gene of the virus was amplified, sequenced and analyzed. Results The complete nucleotide sequence of the S gene of WCL strain was composed of 1698 nucleotides with TA-rich. The S gene contained one ORF, starts at position 37 and ends at position 1326, encoding for the N protein of 429 amino acid residues, and in line with Hantaan ( HTN) -based coding. Sequence comparison showed that WCL was more homologous to HTN viruses than the other hantaviruses, most similar to Bao14 isolated from Apodemus agrarius in Heilongjiang Province, and the amino acid sequence of N protein of WCL has the conserved amino acid sites of HTN viruses. Phylogenetic analysis showed that WCL was clustered into the group of HTN type viruses and was more similar to Heilongjiang Bao14 strain and HTN261 strain than to others, and belonged H4 subtype. So the homology and phylogenetic analysis reflected a certain degree of geographical aggregation characteristic of hantavirus. Conclusion WCL belonged the H4 genetic subtype of HTN viruses with Bao14 and HTN261.