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用黄瓜耐弱光品系M22和弱光敏感品系M14杂交衍生的152个F2单株为作图群体,利用21个SSR多态性标记和135个SRAP多态性标记构建遗传图谱,结合2008年秋季和2009年春季弱光胁迫下123株F2:3家系(F2代单株衍生为F2:3的家系)的下胚轴性状进行QTL定位分析。结果在两季共检测到15个控制下胚轴性状的QTLs,贡献率在5.5%～19.8%,分别定位在LG1、LG2、LG4、LG6和LG7连锁群上。2008年秋季分别检测到6个控制下胚轴长和2个控制下胚轴粗的QTLs,总贡献率分别为58.2%和27.7%;2009年春季分别检测到4个控制下胚轴长和3个控制下胚轴粗的QTLs位点,总贡献率分别为34.6%和34.7%。
Fifty-two F2 individuals derived from cucumber-resistant weak-light M22 and low-light-sensitive M14 hybrids were used as mapping populations. Genetic maps were constructed using 21 SSR polymorphism markers and 135 SRAP polymorphic markers, And the hypocotyl traits of 123 F2: 3 pedigrees (F2 generation derived F2: 3) under low light stress in spring 2009 were analyzed by QTL mapping. Results A total of 15 QTLs controlling hypocotyls were detected in the two seasons with the contribution rate ranging from 5.5% to 19.8%, which were mapped on LG1, LG2, LG4, LG6 and LG7 linkage groups respectively. Six QTLs controlling hypocotyls length and two hypocotyls were detected in the fall of 2008 with the total contribution rates of 58.2% and 27.7%, respectively. Four hypocotyls and three hypocotyls were detected in the spring of 2009 The total number of QTLs controlling the hypocotyls was 34.6% and 34.7%, respectively.