我们在研究小鼠肝脏免疫抑制蛋白的生物学活性、理化特性和纯化的基础上,对人肝免疫抑制蛋白的生物学作用进行了研究.取自死亡在12小时内无明显器质性病变的人肝,充分洗去血液,制成匀浆,经低速、高速、超速离心,其上清液经40～70%饱和硫酸铵沉淀,获得粗的部分纯化肝脏免疫抑制蛋白(PLISP).用~3H 胸苷掺入法观察PLISP 对促有丝分裂原刺激的淋巴细胞增生反应和肿瘤细 We studied the biological effects of human hepatic immunosuppressive proteins on the basis of studying the biological activity, physicochemical properties, and purification of mouse hepatic immunosuppressive proteins. No biological changes were observed within 12 hours after death. Human liver, fully washed blood, made homogenate, after low-speed, high-speed, ultracentrifugation, the supernatant was precipitated with 40 ~ 70% saturated ammonium sulfate to obtain a crude partially purified liver immunosuppressive protein (PLISP). 3H thymidine incorporation assay for PLISP stimulation of mitogen stimulated lymphocyte proliferation and tumor fines