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以吸附率为指标,通过单因素试验比较了5种不同的树脂类型、p H值、溶质浓度和树脂量对ε-聚赖氨酸(ε-PL)的吸附效果,进一步采用响应面设计优化了HZD-3B和D155树脂的吸附条件,分析了两种树脂吸附ε-PL的吸附等温线以及吸附动力学曲线。结果表明,试验的5种树脂中,HZD-3B和D155树脂的吸附效果较好。HZD-3B最佳静态吸附条件为:溶液起始p H 8.5、起始浓度40g/L、树脂用量150g/L、温度25℃、吸附13h,ε-PL的最大吸附率是97.69%;D155最佳静态吸附条件为:溶液起始p H 8、起始浓度40g/L、树脂用量150g/L、温度25℃、吸附14h,ε-PL的最大吸附率是96.84%;等温线数据拟合均较符合Freundlich方程,动力学数据拟合均较符合二级动力学方程。发酵液验证试验结果显示,ε-PL最大吸附量与纯品吸附量基本一致。
Adsorption rate as an index, the adsorption effect of ε-polylysine (ε-PL) by five different resin types, p H value, solute concentration and resin amount was compared by single factor test and further optimized by response surface design The adsorption conditions of HZD-3B and D155 resin were analyzed. The adsorption isotherms and adsorption kinetics curves of the two resins were analyzed. The results showed that the adsorption of HZD-3B and D155 resin was better among the five tested resins. The best static adsorption conditions for HZD-3B were as follows: the initial pH of the solution was p H 8.5, the initial concentration was 40 g / L, the resin dosage was 150 g / L, the maximum adsorption rate of ε-PL was 97.69% The best static adsorption conditions were as follows: the initial pH of the solution was p H 8, the initial concentration was 40 g / L, the resin dosage was 150 g / L, the maximum adsorption rate of ε-PL was 96.84% Compared with the Freundlich equation, the kinetic data fitted well to the second-order kinetic equation. Fermentation broth validation test results show that, ε-PL maximum adsorption capacity and pure adsorption capacity is basically the same.