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目的:研究SV40病毒转化的人角朊细胞系在体外长期培养过程中的细胞生物学特征.方法:采用细胞培养法观察转化细胞系的克隆形成率、细胞生命曲线以及血清、Ca2+对其生长分化的影响.结果:与正常角朊细胞相比,转化角朊细胞系于体外形成克隆所需的最低接种密度有所降低,但接触抑制特性仍未丧失.转化细胞在体外已传代培养25代,对血清的依赖性降低,可被Ca2+诱导分化而形成细胞膜片结构.结论:本研究所观察的转化细胞系可在体外长期传代培养,并保留了正常角朊细胞的部分生物学特征,该细胞系在创面修复研究中具有一定价值.
OBJECTIVE: To study the cell biology characteristics of SV40-transformed human keratinocyte cell line in long-term culture in vitro. Methods: The cell culture method was used to observe the clonal formation rate, cell life curve and the effect of Ca2 + on the growth and differentiation of the transformed cell lines. RESULTS: The lowest inoculum density required for transformed keratinocytes to form a clone in vitro was reduced compared to normal keratinocytes, but the contact inhibition profile was not lost. Transformed cells have been subcultured in vitro 25 generations, reduced serum dependence, can be induced by Ca2 + differentiation and the formation of cell membrane structure. CONCLUSION: The transformed cell lines observed in this study can be subcultured in vitro for a long time and retain some of the biological characteristics of normal keratinocytes. This cell line has a certain value in wound healing research.