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目的:研究整合素α5、pY397FAK及黏着斑激酶(FAK)在子痫前期胎盘中的表达,并探讨其与子痫前期发病机制的关系。方法:应用免疫组化法对24例子痫前期患者和22例正常孕妇胎盘组织中整合素α5、pY397FAK及FAK进行检测,观察其在各组的分布和表达的差异;实时荧光定量RT-PCR法检测胎盘交界处组织中整合素α5及FAK的mRNA表达。结果:子痫前期组的整合素α5及pY397FAK蛋白较正常组表达强度明显下降,差异有显著性(P<0.05);子痫前期组整合素α5mRNA明显低于正常足月妊娠组,差异有显著性(P<0.05);FAK的蛋白及mRNA在两组孕妇胎盘中的表达无显著性差异(P>0.05)。结论:胎盘滋养细胞整合素α5及pY397FAK磷酸化水平的异常表达,在子痫前期的发病中起着重要作用。
Objective: To investigate the expression of integrin α5, pY397FAK and focal adhesion kinase (FAK) in preeclampsia placenta and its relationship with the pathogenesis of preeclampsia. Methods: The expression of integrin α5, pY397FAK and FAK in placenta of 24 cases of preeclampsia and 22 normal pregnant women were detected by immunohistochemistry. The distribution and expression of integrinα5, pY397FAK and FAK were observed by real-time fluorescent quantitative RT-PCR The mRNA expression of integrin α5 and FAK in the placenta junction was detected. Results: The expression of integrin α5 and pY397FAK in preeclampsia group was significantly lower than that in normal group (P <0.05). The expression of integrin α5 mRNA in preeclampsia group was significantly lower than that in normal term pregnancy group (P <0.05). There was no significant difference in the expression of FAK protein and mRNA between the two groups of placenta (P> 0.05). Conclusion: Abnormal expression of integrin α5 and pY397FAK in placental trophoblasts plays an important role in the pathogenesis of preeclampsia.