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羊草是分布于我国北方地区重要的禾本科牧草,为了保护羊草资源和培育羊草新品种,利用基因枪技术进行了CMO和BADH双基因转化羊草的研究。首先,以1301质粒为基础,构建了均由35S启动子驱动的无抗生素选择标记CMO基因和BADH基因的植物双基因表达载体pCAMBIA-1301-CMO-BADH;再通过基因枪技术将其导入羊草中,PCR扩增后电泳检测及Southern杂交分析表明,外源基因已整合到受体植物基因组中并正常表达。在高浓度混合盐和高pH值胁迫下,转双基因植株甜菜碱含量高于对照植株。
Leymus chinensis is an important gramineous pasture distributed in the northern part of China. In order to protect Leymus chinensis and breed new Leymus chinensis, two genetically modified Leymus chinensis (CMO) and BADH genes were studied by using gene gun technology. First of all, based on the 1301 plasmid, a plant dual gene expression vector pCAMBIA-1301-CMO-BADH without antibiotic selection marker CMO gene and BADH gene driven by 35S promoter was constructed and introduced into Leymus chinensis The results of PCR amplification and Southern hybridization showed that the foreign gene was integrated into the genome of recipient plant and expressed normally. Under the condition of high concentration of mixed salt and high pH stress, the content of betaine in transgenic double transgenic plants was higher than that of the control plants.