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目的:建立了用HPLC对苦豆草中苦豆碱、槐定碱、氧化苦参碱、氧化槐果碱、苦参碱、槐果碱和莱曼碱同时定量的方法。方法:采用HPLC检测方法,色谱柱W aters X-B rige C18(4.6 mm×250 mm,5μm);流动相为乙腈-0.05 mol.L-1的磷酸二氢钾溶液(2.0 mL.L-1三乙胺),进行梯度洗脱;检测波长为205 nm,柱温25℃,进样量10μL,流速1.0 mL.m in-1。结果:苦豆碱、槐定碱、氧化苦参碱、氧化槐果碱、苦参碱、槐果碱和莱曼碱的浓度与峰面积,分别在20.66～103.32μg(r=0.998 8),22.82～114.12μg(r=0.999 7),25.10～125.52μg(r=0.999 1),23.88～119.40μg(r=0.997 5),5.00～24.99μg(r=0.998 6),4.69～23.46μg(r=0.999 6)和4.60～23.01μg(r=0.997 8)范围呈良好的线性关系;平均加样回收率分别为97.12%,97.47%,96.21%,94.64%,98.04%,96.24%和101.31%;RSD分别为7.3%,3.0%,4.5%,5.2%,5.4%,5.8%,4.3%。结论:该方法准确、简便,灵敏,为苦豆草的质量评价提供了依据。
OBJECTIVE: To establish a method for simultaneous quantitation of alperitine, sophoridine, oxymatrine, oxycinnamine, matrine, sophocarpine and Lemanine in Sophora alopecuroids by HPLC. Methods: The HPLC method was used. The mobile phase consisted of acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (2.0 mL.L-1 triethylenetetramine Amine). The detection wavelength was 205 nm and the column temperature was 25 ℃. The injection volume was 10 μL and the flow rate was 1.0 mL.m in-1. Results: The concentrations and peak areas of alpericarb, sophoridine, oxymatrine, oxycinnamine, matrine, sophocarpine and Lemanine were 20.66 ~ 103.32μg (r = 0.998 8) 22.82-114.12 μg (r = 0.999 7), 25.10-125.52 μg (r = 0.999 1), 23.88-119.40 μg (r = 0.997 5), 5.00-24.99 μg (r = 0.998 6), 4.69-23.46 μg = 0.999 6) and 4.60 ~ 23.01μg (r = 0.997 8). The average recoveries were 97.12%, 97.47%, 96.21%, 94.64%, 98.04%, 96.24% and 101.31%, respectively. RSD were 7.3%, 3.0%, 4.5%, 5.2%, 5.4%, 5.8%, 4.3% respectively. Conclusion: The method is accurate, simple and sensitive and provides the basis for the quality evaluation of Sophora japonica.