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2010年,作者在辽东半岛成功进行了裙带菜室内常温全人工育苗大规模生产试验,并全程进行了光照强度、温度和配子体发育相关性的研究。研究表明,采用双高光期(≥50μmolphotons/m2·s)调控配子体的生长和发育可达到理想效果。从采苗至度夏前,采用高光照使配子体充分完成营养生长;从度夏结束至幼苗出库前,同样采用高光照,使配子体快速发育形成孢子体并迅速生长。现场观察表明,只要光照强度逾越了诱导发育的阈值,裙带菜配子体在24℃仍然能够正常发育并形成孢子体;幼孢子体能够耐受24～26℃的高温,但是最适生长温度为20～22℃;孢子体越小,耐受高温能力越强。在24～26℃的高温条件下,幼孢子体生长缓慢,较大个体会出现尖端溃烂缺失的现象。在整个育苗过程中,根据温度变化和配子体的显微观察结果因地制宜地对光线进行实时调整。出库前培育的苗帘在幼苗密度、大小和健康方面均达到商业化栽培的要求。
In 2010, the author successfully carried out the large-scale production experiment of artificial artificial nursery seedlings at room temperature in the wakame seaweed in Liaodong Peninsula. The correlation between light intensity, temperature and gametophyte development was also studied. Studies have shown that the use of double-light (≥ 50μmolphotons / m2 · s) regulation of gametophyte growth and development can achieve the desired effect. From the beginning of seedling to the summer, the gametophytes were fully vegetated by high light. From the end of summer to the seedling exiting, the same high light was used to make the gametophyte grow rapidly to form sporophytes and grow rapidly. Field observations showed that as long as the light intensity exceeded the threshold for inducing development, the gametophyte of Undaria pinnatifida could still normally develop and form sporophytes at 24 ° C. The young sporophytes tolerated the high temperature of 24-26 ° C, but the optimum growth temperature was 20-30 ° C. 22 ℃; sporophyte smaller, more resistant to high temperature ability. Under the condition of high temperature from 24 to 26 ℃, the sporophytes grow slowly, and the larger individuals have the phenomenon of lacking of the tip fester. Throughout the nursery process, according to temperature changes and microscopic observations of gametophyte light conditions for real-time adjustment. Seedlings nurtured before leaving the library in seedling density, size and health have reached the commercial cultivation requirements.