论文部分内容阅读
目的:探讨地五养肝胶囊(DWYG)对肝纤维化大鼠肝脏Hedgehog(Hh)信号通路的影响。方法:Wistar大鼠随机分为空白对照组、肝纤维化模型组和DWYG干预组,四氯化碳(CCl4)诱导肝纤维化模型。应用HE和Masson染色检测不同处理组大鼠肝组织损伤和纤维化程度;应用Western Blot和Real-time q RT-PCR分别检测不同处理组大鼠肝组织Hh信号通路的蛋白和基因表达水平。结果:与空白对照组相比,肝纤维化模型组大鼠出现明显肝组织损伤和纤维化改变,且纤维化肝组织中Shh、Gli1、Smo和Ptc的蛋白和基因表达水平显著上调(P<0.05);而与肝纤维化模型组相比,DWYG干预可显著减轻肝组织损伤和纤维化程度,同时显著下调纤维化肝组织中Shh、Gli1、Smo和Ptc的蛋白和基因表达水平(P<0.05)。结论:DWYG抗纤维化效应与其抑制纤维化大鼠肝组织中过度活化的Hh信号通路相关。
Objective: To investigate the effect of DiYangGanGui Capsule (DWYG) on hepatic Hedgehog (Hh) signal pathway in hepatic fibrosis rats. Methods: Wistar rats were randomly divided into blank control group, hepatic fibrosis model group and DWYG intervention group. Carbon tetrachloride (CCl4) induced hepatic fibrosis model. HE staining and Masson staining were used to detect liver tissue injury and fibrosis in different groups. Western Blot and Real-time q RT-PCR were used to detect the protein and gene expression of Hh signaling pathway in different groups. Results: Compared with the blank control group, the liver injury and fibrosis in the liver fibrosis model group were significantly changed, and the protein and gene expression levels of Shh, Gli1, Smo and Ptc in the fibrosis liver tissue were significantly increased (P < 0.05). Compared with the liver fibrosis model group, DWYG treatment could significantly reduce the degree of liver injury and fibrosis, and significantly reduce the protein and gene expression of Shh, Gli1, Smo and Ptc in fibrosis liver tissue (P < 0.05). CONCLUSION: The anti-fibrotic effect of DWYG is related to Hh signaling pathway that inhibits over-activation in hepatic tissue of fibrotic rats.